2-photon microscope SUPERNOVA-100

opticallaboratoryfluorescence

Complete Solutions for in vivo Imaging, Revolutionising Neuroscience Research! Imaging neurons and synapses in the brain of free-moving animals with the resolution of a benchtop two-photon microscope, providing neuroscientists with a revolutionary new tool and opening up a new paradigm of neuroscience research. Small: 2.6g Headpiece The headpiece is designed to provide distortion-free conduction of femtosecond laser pulses, high-speed scanning, high-efficiency fluorescence excitation and collection, empowering high-resolution imaging of brain neurons and synapses in freely behaving animals. Applications The large-field two-color miniature two-photon microscope combined with the optogenetic module were used to observe overall network activity of dorsomedial prefrontal cortex (dmPFC) after inhibiting PV rmlrons or VIP rmlrons. By optogenetic manipulating and calcium imaging of specific neurons, the neuronal mechanism behind the "winner effect" was revealed. Study of the itch perception in freely behaving animals requires minimal input of stimulus The miniature two-photon microscopy enables neuronal calcium imaging for itch study. Itch was induced by manipulation of GRPR neurons in spinal cord, and activity of SITr neurons was recorded while mouse scratching. Fluorescence of genetically fluorescent acetylcholine indicator (ACh3.0) was recorded using a miniature two-photon microscope while mouse running, to image the neurotransmitters in real-time in freely animals.