Process bioreactor

automated

This bioreactor line is for the cultivation of Pichia pastoris to manufacture enzymes and proteins. The system is fully closed and automated, consisting of following key elements: 50, 500 and 2×5000 litre bioreactors (total volumes); Stationary CIP/SIP for automated cleaning and sterilization of bioreactors, reactors and connecting pipelines; Circulation sterilizer for sterilization of substrate; Feeding reactors for glycerol and methanol; Connecting heat-insulated pipelines and the group of pneumatically controlled membrane valves. The following parameters in bioreactors are controlled: • temperature; • pH; • DO; • overpressure; • hydrostatic pressure; • level; • methanol concentration; • foaming. The central cascade control system includes SCADA developed in compliance with the requirements of 21 CFR Part 11.