At the heart of the HSi-300 Hyperspectral Imaging System is our patented acousto-optical tunable filter (AOTF) imaging module. This technology fulfills the promise of multispectral and hyperspectral imaging through unmatched spectral flexibility and switching speed. The HSi-300 is now available with µ-Manager plug-ins, a free and open source application software platform for imaging and control of automated microscopes on multiple operating systems (Windows, Mac and Linux). The hyperspectral imaging systems are also currently integrated with DVC cameras and the Andor Ixon EMCCD family of scientific cameras, and will soon be integrated with Hamamatsu and Photometrics.