The Biolit 2 is a compact, air-cooled femtosecond laser specifically designed for multiphoton
microscopy, non-linear spectroscopy, and other biophotonics applications. With an average
power of 2 W and pulse energy on the order of 100 nJ in the NIR spectral range (1050 nm) is
sufficient for most optical imaging applications. The beam quality with M 2 <1.2 (typ.1.05) and
circularity >0.9 (typ. 0.94) is superb among the leading market players and fits the most demanding
requirements of novel living tissue imaging techniques. The exceptional beam quality,
characterized by an M 2 value of <1.2 (typically 1.05) and circularity greater than 0.9 (typically
0.94), is superb among the leading market players and meets the most demanding
requirements of cutting-edge living tissue imaging techniques.
The emitted optical pulses feature a Gaussian-like shape with almost no modulations and
pedestal. To benefit from remarkably short pulses of <70 fs (typ. 55 fs) when using them with
high numerical aperture thick objectives, the laser incorporates a dispersion pre-compensation
feature. Offering standard pulse repetition rates of 15, 20, 30, or 40 MHz, with the flexibility of
extending this range to 1-20 MHz, users can fine-tune the pulse repetition rate to suit specific
sample characteristics and achieve the strongest usable signal with the best signal-to-noise ratios.
The Biolit-2 SH model is equipped with a built-in harmonics module that delivers 0.4 W at
a wavelength of 525 nm. This wavelength extension makes it an excellent choice for
photopolymerization, a cutting-edge 3D printing method renowned for its exceptional sub-100
nm resolution capabilities.