Storage Temperature:
Stored at room temperature for three years.
Product Introduction:
Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been
removed after purification. Proteinase K is active in the presence of SDS or urea as well as in a
wide range of pH, salt concentration and temperature, and can be increased in 1% SDS.
Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA,
western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the
specific working concentration is determined according to whether the buffer solution
contains SDS, urea, pH, temperature and other factors.
RNase and DNase are not included;
Specs:1g;
Active in denaturant, such as urea and SDS;
Active in metal chelators, such as EDTA;
Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0;
Active in a wide range of temperature, generally operating temperature range of 50℃-65℃;
ddH2O can be dissolved;
7.Enzyme activity value: ≥30 U/mg Lyophilized powder.
Method of Inactivation:
After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reactionsystem, it was deactivated.