Ultra-fast liquid chromatograph Nexera UFPLC

processpreparativeon-line

When developing new drugs, structural analysis of impurities and metabolites is a critical process that cannot be avoided. However, structural analysis after synthesis or after purification by preparative LC is time consuming and recovering high-purity samples is very difficult. Therefore, as a solution to this problem, Shimadzu offered the Crude2Pure system, which recovers target compounds in high-purity powder based on a revolutionary purification method that involves using two purification systems. The Ultrahigh-speed Preparative Purification System, Nexera UFPLC system, the newest addition to Shimadzu's family of purification systems, inherits the same purification method as the Crude2Pure, but handles all steps, from fractionation to purification, within a single system, so that target compounds can be recovered in high-purity solution. With conventional preparative methods, the fractionated sample contains mobile phase solvent, which leads to unavoidable dilution during fractionation, so the fractionated sample must be enriched and desalted. Such procedures complicate the workflow, and amplify sample loss, labor, and costs. With the Shimadzu UFPLC Ultra Fast Preparative and Purification Liquid Chromatograph, all the work from preparation to enrichment, purification, and recovery is performed on-line. This significantly reduces the labor involved in the preparative purification process, and avoids procedural mistakes. Using a proprietary trap enrichment purification technology for the fractionated target components, trace quantities of components contained in mixtures can be recovered at high concentrations and high purity levels.