Spray system iMLayer AERO

The iMLayer™ AERO incorporates a sample stage that moves at a controlled rate while maintaining the same distance from the spray nozzle, enabling stable matrix spraying. Over multiple strokes, the sample becomes laminated with fine matrix crystals, enabling high sensitivity and high spatial resolution. Humidity does not impact matrix deposition because the atmosphere within the spray chamber is replaced before pretreatment. Spraying can be performed under more stable conditions than with a hand spray. If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. The rinsing mechanism allows for clog-free, stable spraying, which enables high reproducibility in MALDI analyses. The standard protocol provides sensitivity on par with a hand spray along with improved resolution, homogeneity and reproducibility To acquire high spatial resolution (5 to 10 μm) images and increase sensitivity, a two-step vapor deposition method can be implemented in combination with the iMLayer matrix vapor deposition system has been developed.* This unique experiment can only be implemented using Shimadzu sample preparation solutions.