Liquid chromatograph Vanquish™ Trap Heart-Cut

laboratoryprocesstwo-dimensional

Achieve in-depth analysis for greater confidence in peak purity and identity with the Thermo Scientific Vanquish Trap Heart-Cut 2D-LC System. This two-dimensional LC (LC-LC) system enables you to collect a single fraction of the first-dimension separation onto a trap column to pre-concentrate, and/or achieve mobile phase compatibility for transferring the fraction for a second-dimension separation or compatibility between UV and MS detection. Heart-cut 2D-LC can reveal hidden impurities that co-elute with the analytes of interest by applying two complementary column chemistries to gain more separation power. The system is configured using modules from the Vanquish Flex UHPLC or Horizon UHPLC systems, delivering performance, robustness and ease of use of the Vanquish LC platform. Greater confidence Collect single or multiple fractions of the first-dimension separation onto a trap column and transfer the cut-out fraction(s) to a second-dimension column with complementary column chemistry in an automated workflow for more confidence in peak purity. Increased sensitivity The trap column enables enrichment of low-abundant analytes and removal of diluting or disturbing solvents from the eluent by trapping and pre-concentrating the analytes of interest to increase sensitivity. Simplified method development The second-dimension eluent provides the flow for solvent modulation, such as eluent strength reduction or buffer exchange of the first-dimension fraction. This process can address solvent incompatibility of UV and MS methods or mobile phase incompatibility between different column chemistries to support multiple combinations of 1D and 2D methods.